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anti cd49f  (Thermo Fisher)


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    Structured Review

    Thermo Fisher anti cd49f
    Anti Cd49f, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd49f/ESTRIOL/us12516024-545-42-43
    Average 94 stars, based on 1 article reviews
    anti cd49f - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Skin graft with dermis and appendages generated in vivo by cell competition.
    Article Snippet: 17105-041) at 4 °C, 12–24 h. Epidermis isolated from the skin was digested with accutase (Innovative Cell Technologies, California, USA, Catalog No. AT-104) to prepare single-cell suspensions. .. Samples were stained with anti-CD45 (1:100, clone 30-F11, Biolegend, Catalog No. 103126), anti-CD117 (1:100, clone 2B8, eBioscience, Catalog No. 105814), and anti-CD49f (1:100, clone GoH3, eBioscience, Catalog No. 17-0495-82) antibodies for 30min on ice. .. Spleens were dissociated into single cells by pipetting, hemolyzed in ACK buffer, and stained with anti-CD45 antibody (1:100, clone 30-F11, eBioscience, Catalog No. 17-0451-82) for 30min.

    Article Title: KDM4 inhibitors
    Article Snippet: Following drug exposure, 4×105 cells were collected and stained with 50 μL propidium iodide (PI) using the PI/RNase solution (Cell Signaling Technol. .. 1×105 cells were washed with FACS buffer (PBS+1% BSA) and stained for 20 min at room temp in the dark with the following antibodies diluted in FACS buffer: anti-CD24 (eBioscience, 46-0247; 1:100), anti-CD44 (eBioscience, 12-0441-81; 1:1000), anti-EpCAM (eBioscience, 660 50-9326; 1:100), and anti-CD49f (eBioscience, 46-0495; 1:200). .. Cells were analyzed using BD LSR Fortessa and BD FACS Diva Software (Becton Dickinson).

    Article Title: Skin graft with dermis and appendages generated in vivo by cell competition
    Article Snippet: The skin was harvested from p63 knockout chimera and WT-chimera and digested in CnT-07 medium supplemented with 25 U/ml dispase (Gibco, Life Technologies, Carlsbad, CA, Catalog No. 17105-041) at 4 °C, 12–24 h. Epidermis isolated from the skin was digested with accutase (Innovative Cell Technologies, California, USA, Catalog No. AT-104) to prepare single-cell suspensions. .. Samples were stained with anti-CD45 (1:100, clone 30-F11, Biolegend, Catalog No. 103126), anti-CD117 (1:100, clone 2B8, eBioscience, Catalog No. 105814), and anti-CD49f (1:100, clone GoH3, eBioscience, Catalog No. 17-0495-82) antibodies for 30 min on ice. .. Spleens were dissociated into single cells by pipetting, hemolyzed in ACK buffer, and stained with anti-CD45 antibody (1:100, clone 30-F11, eBioscience, Catalog No. 17-0451-82) for 30 min. Each sample was stained with Propidium iodide (PI) (Sigma-Aldrich, St. Louis, MO, Catalog No. P4170) or 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Carlsbad, CA, Catalog No. D1306) and analyzed by CytoFLEX S (B75442, BECKMAN COULTER, California, USA).

    Article Title: Chemotherapeutic Stress Influences Epithelial–Mesenchymal Transition and Stemness in Cancer Stem Cells of Triple-Negative Breast Cancer
    Article Snippet: .. To analyze the expression of surface markers, approximately 1 × 10 5 cells were washed with staining buffer (DPBS + 1% bovine serum albumin (BSA, Carl Roth, 8076.2, Karlsruhe, Germany)) and stained for 20 min at room temperature in the dark with the antibodies diluted in staining buffer as below: Anti-CD24 (eBioscience, Bleiswijk, Netherlands, 46-0247; 1:100), anti-CD44 (eBioscience, Bleiswijk, Netherlands, 12-0441-81; 1:1000), anti-EpCAM (eBioscience, Bleiswijk, Netherlands, 660 50-9326; 1:100), and anti-CD49f (eBioscience, Bleiswijk, Netherlands, 46-0495; 1:200). .. Cells were analyzed using BD LSRFortessa TM cell analyzer (BD, San Jose, CA, USA) and FlowJo software (Version 6, Ashland, OR, USA).

    FACS:

    Article Title: KDM4 inhibitors
    Article Snippet: Following drug exposure, 4×105 cells were collected and stained with 50 μL propidium iodide (PI) using the PI/RNase solution (Cell Signaling Technol. .. 1×105 cells were washed with FACS buffer (PBS+1% BSA) and stained for 20 min at room temp in the dark with the following antibodies diluted in FACS buffer: anti-CD24 (eBioscience, 46-0247; 1:100), anti-CD44 (eBioscience, 12-0441-81; 1:1000), anti-EpCAM (eBioscience, 660 50-9326; 1:100), and anti-CD49f (eBioscience, 46-0495; 1:200). .. Cells were analyzed using BD LSR Fortessa and BD FACS Diva Software (Becton Dickinson).

    Expressing:

    Article Title: Chemotherapeutic Stress Influences Epithelial–Mesenchymal Transition and Stemness in Cancer Stem Cells of Triple-Negative Breast Cancer
    Article Snippet: .. To analyze the expression of surface markers, approximately 1 × 10 5 cells were washed with staining buffer (DPBS + 1% bovine serum albumin (BSA, Carl Roth, 8076.2, Karlsruhe, Germany)) and stained for 20 min at room temperature in the dark with the antibodies diluted in staining buffer as below: Anti-CD24 (eBioscience, Bleiswijk, Netherlands, 46-0247; 1:100), anti-CD44 (eBioscience, Bleiswijk, Netherlands, 12-0441-81; 1:1000), anti-EpCAM (eBioscience, Bleiswijk, Netherlands, 660 50-9326; 1:100), and anti-CD49f (eBioscience, Bleiswijk, Netherlands, 46-0495; 1:200). .. Cells were analyzed using BD LSRFortessa TM cell analyzer (BD, San Jose, CA, USA) and FlowJo software (Version 6, Ashland, OR, USA).



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